Validation of autophagy targeting drugs in neurons and increased peroxidated lipids in neurons treated with HBSS. (A and B) Neurons ± bafilomycin A1 (BAF A1), bafilomycin B1 (BAF B1), chloroquine (CQ), 3-Methyladenine (3-MA), or erastin in HBSS were analyzed by Western blot for LC3 and β-actin. n = 4–6 independent experiments; mean ± SD. One-way ANOVA with Tukey’s post test. (C) Confocal maximum intensity projection of neurons in media or HBSS ± DMSO or ferrostatin-1 labeled with BD-C11. Non-peroxidated (BD-C11 non-ox) and peroxidated (BD-C11-ox) lipids. Scale bars, 10 μm. (D) Quantification of lipid peroxidation (ratio BD-C11 ox/ BD-C11 non-ox) in neurons in media or HBSS ± DMSO or ferrostatin-1. n = 4 independent experiments; mean ± SEM. One-way ANOVA with Tukey’s post test. (E) Airyscan image of live neuron in HBSS labeled with BD-C11 and LysoTracker Blue. Non-peroxidated (BD-C11 non-ox) and peroxidated (BD-C11 ox) lipids. Boxed area magnified in left panels. Scale bar, 5 μm. Source data are available for this figure: SourceData FS3.