Lipid circuits activated in response to edelfosine. (A) Lipid transport and metabolic pathway map highlighting upregulated (green dots) and downregulated (red dots) genes detected by RNA-seq. Color code reflects the fold change as indicated in the figure. (B) Volcano plot of transcripts changed in response to edelfosine as identified by the RNA-seq transcriptome analysis. Targets of Opi1/Ino2/4 are colored red. Targets of Spt23 are colored green. Genes confirmed by qPCR (inset) are labeled. For the inset, bars represent mean ± SD for three independent experiments while circles represent individual experiments.