Genetic background and gene ontology analysis of RNA-seq hits. (A) Scatter plots of the first three principal component analysis scores for the transcriptional landscape of each sample submitted for RNA-seq. W303 samples are represented as circles while BY4741 samples are represented as triangles. Large circles indicate the mean PC score for each treatment. Red is Ctrl, and blue is EDLF. (B) Molecular function GO terms enriched in genes downregulated (left) and upregulated (right) in edelfosine more than ln(1.5-fold). Legend demonstrating correlation of size and color with the number of genes per term and the significance score, respectively, is in the middle. (C) Schematic of rDNA repeats showing regions amplified by qPCR primers. (D) qPCR of 35S rDNA targets (P2-4) in WT cells treated for 60 min with edelfosine or vehicle expressed as ln(EDLF/Ctrl) and normalized to IGS2. Bars represent mean ± SD for three independent experiments while circles represent individual experiments. For all panels: Ctrl = vehicle; EDLF = edelfosine.