Figure S2. Telomere interactions with... | Rockefeller University Press

Figure S2.

$Telomere interactions with the nuclear membrane are preserved in edelfosine. (A) Representative cells expressing the Tel06R constructs quantified in Fig. 2 B were imaged using live fluorescence microscopy after 90 min in edelfosine or the vehicle. Scale bar represents 2 µm. (B) Membrane and soluble fractions were collected from BY4741 cells expressing Spt23-TAP treated with 20 μM edelfosine or vehicle for 60 min as described in Materials and methods, then blotted for detection of endogenous Rap1 and Pgk1. (C) Cell cycle analysis of wild-type cells (W303) treated with edelfosine or vehicle for the indicated times. For all panels: Ctrl = vehicle; EDLF = edelfosine. Source data are available for this figure: SourceData FS2.$

Telomere interactions with the nuclear membrane are preserved in edelfosine. (A) Representative cells expressing the Tel06R constructs quantified in Fig. 2 B were imaged using live fluorescence microscopy after 90 min in edelfosine or the vehicle. Scale bar represents 2 µm. (B) Membrane and soluble fractions were collected from BY4741 cells expressing Spt23-TAP treated with 20 μM edelfosine or vehicle for 60 min as described in Materials and methods, then blotted for detection of endogenous Rap1 and Pgk1. (C) Cell cycle analysis of wild-type cells (W303) treated with edelfosine or vehicle for the indicated times. For all panels: Ctrl = vehicle; EDLF = edelfosine. Source data are available for this figure: SourceData FS2.