The metabolically stable lysophosphatidylcholine analogue edelfosine alters nuclear envelope morphology and nuclear architecture. (A) Representative images of wild-type (W303) cells expressing the ER marker Sec63^GFP from a centromeric plasmid. Cells were imaged using live fluorescence microscopy after growth in SD-Leu+Ade and 90 min in the presence of edelfosine or the vehicle. Quantification of cells displaying abnormal nuclear membrane morphology is shown beside the microscopy images. Circles represent the percentage of cells displaying abnormal nuclear morphology in each experiment (two-sided Fisher’s exact test gave a P value <0.0001 for each experiment [n = 100 cells per treatment]). Bars represent the mean of the three independent experiments ± SD. ** indicates a P value <0.01 as determined by two-tailed unpaired t test (N = 3). (B) Representative images of wild-type (W303) cells expressing the Nop1^CFP nucleolar marker expressed from a centromeric plasmid in cells with Nup49^GFP (nuclear envelope marker) endogenously tagged. Cells were imaged using live fluorescence microscopy after growth in SD-Ura+Ade and 90 min in the presence of edelfosine or the vehicle. The distribution of values from the quantification of nucleolar volume of cells (described in Materials and methods) is shown beside the microscopy images. Values cumulative from three independent experiments (n = 163 Ctrl, 190 EDLF) are shown as small circles, with the means of each experiment shown as large circles. Bars represent the mean with 95% confidence interval from the pooled values. **** indicates a P value <0.0001 as determined by two-tailed nested t test (N = 3). For all panels: Ctrl = vehicle; EDLF = edelfosine; scale bars represent 2 µm.