To generate samples for transmission electron microscopic analysis of retained cytoskeleton in Δcsar1 vacuoles (see Fig. 5 ), infected cells were first imaged by fluorescence (GFP-tubulin) and DIC to identify regions of interest. Multiple slices were then mounted on EM grids. Shown is an additional slice in this region mounted on a slide for visualization by DIC. Red arrowheads indicate landmark features. Yellow arrowhead indicates the cell infected with four vacuoles of Δcsar1 parasites that was imaged and shown in Fig. 5.