CSAR1 is excluded from daughter buds. (A) 0.5 μm slice through a budding CSAR1^3xHA parasites from a confocal stack. CSAR1^3xHA (magenta), while building up in the maternal cytosol, appears excluded from the cytosol of daughter buds, which are outlined by GFP-tubulin (green). Scale bar is 5 μm. (B) A line scan at the indicated position in A highlights the lack of CSAR1^3xHA signal within a daughter bud.