CSAR1 mislocalizes to daughter buds upon ERK7 degradation. (A and B) Maximum intensity Z-projections of confocal stacks of CSAR1-3xHA ERK7^AID parasites grown for 24 h (A) without IAA and (B) with IAA to degrade ERK7^AID. Parasites were visualized with GFP-tubulin (blue), and antibodies against HA (green) and the apical cap marker ISP1 (red). Concentration of CSAR1 signal at the residual body is indicated with a white arrow. The apical ends of the late daughter buds in the + IAA condition are indicated with orange arrows. Parasites were captured at the indicated points in the cell cycle. (C) Maximum intensity Z-projects of confocal stack of CSAR1-3XHA ERK7^AID parasites counterstained with an anti-ERK7 antibody. All scale bars are 10 μm.