Candidates from the ERK7 interactome were examined by creating screens in which the gene of interest was expressed in-frame with a C-terminal 3xHA tag in the background of the ERK7 ^AID strain. Parasites were grown in ±IAA for 24 h before fixation. Samples were stained with antibodies recognizing the HA-tag (green), tubulin (blue), and the indicated counter-stain (red; usually ISP1, a marker for the apical cap; in some cases IMC1 a marker for the inner-membrane complex; ROP2 a marker for the rhoptries; MIC2 a marker for the micronemes; GRA2 a marker for secretion into the parasitophorous vacuole). For unmerged images, see preprint (O’Shaughnessy et al., 2023). All images are representative of a minimum of n = 20 images and are maximum intensity Z-projections of confocal stacks. While TGGT1_236560 was a hit in both our Yeast-two-hybrid and BioID datasets, C-terminal tagging led to vacuole localization, which is inconsistent with an interaction with cytosolic ERK7. TGGT1_236560 also has no predicted signal peptide. Note that the predicted protein length is ∼250 kD, and the gene has no predicted introns, which is unusual for a gene of this size. It is therefore possible that the gene model is incorrect and represents a combination of a cytosolic protein (at the 5′ end of the gene) and a secreted protein (at the 3′ end).