cadm3-ig1De ⁺ but not cadm3 ⁺ RPCs produce expanded clones in WT host retinae. (A) Representative images showing neurogenesis wave in WT and cadm3-ig1De⁺ retinae by SoFa fish line. Areas within white dashed contour lines are retinae. (B) Representative clones derived from single 12-hpf WT, cadm3-ig1De⁺ (n = 24), or cadm3⁺ (n = 19) donor RPCs in WT host retinae. OPL, the outer plexiform layer; IPL, the inner plexiform layer. (C) Quantification of the size of 72-hpf clones in B. Data in C represent mean ± SEM. (D) A representative image showing the distribution of Cadm3-GFP fusion protein on the membrane of cadm3-overexpressing donor RPCs in WT host retinae. The orange dashed rectangle circles a region (1) of the membrane between one cadm3-overexpressing donor RPC and one WT host RPC. The yellow dashed rectangle circles a region (2) of the membrane between two cadm3-overexpressing RPCs. Zoom-in images of region 1 and region 2 are shown in the under panel. (E) Quantification of the mean gray value of membrane GFP in region 1 and region 2 using ImageJ software. (F) Images showing Cadm3-GFP expression on the cell membrane in the 28- and 34-hpf retinae. Areas between two dashed white lines are retinae in D and F. Scale bars, 10 µm.