Figure 7.
Cadm3 failed in homophilic interaction could drive the clonal expansion of RPCs. (A and B) Representative images (A) and quantification of clone size (B) of 72-hpf clones in WT retinae (n = 26) or retinae with cadm3-ig1De expression (n = 27). Inserts (top) in A describe the patterns of Cadm3-mediated cell–cell interaction in different conditions. OPL, the outer plexiform layer; IPL, the inner plexiform layer. (C and D) Representative cases (C) and quantification of cell-cycle length (D) of single 12-hpf WT (top, n = 34) and cadm3-ig1De+ (bottom, n = 22) RPCs. The area of dashed white circles is the optic vesicle, and the area between two dashed white lines is the retina. Yellow arrowheads show RPCs at the M phase. (E) Representative DAPI staining images showing the most central coronal sections with the optic nerve of 72-hpf WT (gfp mRNA injection control, top, n = 13) and cadm3-ig1De+ (bottom, n = 18) retinae. Yellow dashed contour lines show 30° sectors centered around the center of the lens beside the optic nerve. Areas within white dashed contour lines are the retinae. (F) Quantification of total cell number (DAPI+) per 30° sector in the coronal sections of WT and cadm3-ig1De+ retinae. (G) Summary schematic describing Cadm3-mediated cell competition model. Data in B, D, and F represent mean ± SEM. Scale bars, 10 µm.

Cadm3 failed in homophilic interaction could drive the clonal expansion of RPCs. (A and B) Representative images (A) and quantification of clone size (B) of 72-hpf clones in WT retinae (n = 26) or retinae with cadm3-ig1De expression (n = 27). Inserts (top) in A describe the patterns of Cadm3-mediated cell–cell interaction in different conditions. OPL, the outer plexiform layer; IPL, the inner plexiform layer. (C and D) Representative cases (C) and quantification of cell-cycle length (D) of single 12-hpf WT (top, n = 34) and cadm3-ig1De+ (bottom, n = 22) RPCs. The area of dashed white circles is the optic vesicle, and the area between two dashed white lines is the retina. Yellow arrowheads show RPCs at the M phase. (E) Representative DAPI staining images showing the most central coronal sections with the optic nerve of 72-hpf WT (gfp mRNA injection control, top, n = 13) and cadm3-ig1De+ (bottom, n = 18) retinae. Yellow dashed contour lines show 30° sectors centered around the center of the lens beside the optic nerve. Areas within white dashed contour lines are the retinae. (F) Quantification of total cell number (DAPI+) per 30° sector in the coronal sections of WT and cadm3-ig1De+ retinae. (G) Summary schematic describing Cadm3-mediated cell competition model. Data in B, D, and F represent mean ± SEM. Scale bars, 10 µm.

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