cadm1a and cadm4 play few roles in coordinated RPC proliferation. (A) Representative clones derived from single 12-hpf WT donor RPCs in WT (n = 34), cadm3⁺ (n = 5), and cadm3-ect⁺ (n = 4) host retinae. (B) The quantification of the size of 72-hpf clones in A. (C) Whole mount GFP immunostaining (green) and TUNEL assay (magenta with yellow arrowheads) of 28-hpf WT host retinae transplanted with gfp-expressing p15⁺ (black box on the left, n = 10) or p15⁺/cadm3⁺ (black box on the right, n = 10) donor RPCs. In each group, zoom-in images of retinal areas (dashed yellow rectangles) at the merged and three separate channels are shown in the right panels. Schematic inserts show TUNEL signals (magenta) in p15⁺ and p15⁺/cadm3⁺ donor RPCs (green) in WT host retinae (gray). Areas within white dashed contour lines are retinae. (D) The ratio of the number of GFP+TUNEL+ nuclear fragments in p15⁺ and p15⁺/cadm3⁺ donor RPCs to the total number of GFP+ donor RPCs in WT host retinae. (E) Representative clones derived from single 12-hpf WT donor RPCs in WT, p15⁺, p15⁺/cadm1a⁺ (n = 29), or p15⁺/cadm4⁺ (n = 25) host retinae. (F) Quantification of the size of 72-hpf clones in E. (G) Representative clones derived from single 12-hpf WT donor RPCs in scramble-sg (n = 25), cadm1a-disrupted (n = 28), or cadm4-disrupted (n = 25) host retinae. (H) Quantification of the size of 72-hpf clones in G. Data in B, D, F, and H represent mean ± SEM. Scale bars, 10 µm. OPL, the outer plexiform layer; IPL, the inner plexiform layer.