Differential expressed genes in WT donor and p15 ⁺ host RPCs. (A) Slice GFP immunostaining (green) and TUNEL assay (magenta with yellow arrowheads) of p15⁺ host retinae at 28 hpf transplanted with gfp-expressing WT donor RPCs. Zoom-in images of retinal areas (dashed yellow rectangle) at three separate channels are shown in the right panels. Schematic inserts show TUNEL signals (magenta) in p15⁺ host RPCs (gray) neighboring to WT donor RPCs (green). Areas within white dashed contour lines are retinae. Scale bars, 10 µm. (B) Ratios of the number of TUNEL+ p15⁺ host RPCs per section with the location (distance, measured by soma numbers) relative to the closest GFP+ WT donor RPCs to total TUNEL+ cell number in p15⁺ retinae. Data represent mean ± SEM. (C) GO analysis of highly expressed genes (fold change of FPKM > 3) in p15⁺ host RPCs compared to WT donor RPCs. (D) GO analysis of highly expressed genes (fold change of FPKM > 3) in WT donor RPCs compared to p15⁺ host RPCs. (E) Heatmap of marker genes of four cell states in p15⁺ host (green bar) and WT donor (magenta bar) RPCs at 24 hpf.