Figure S1.
p15+hosts induce faster proliferation of WT donor RPCs at the early proliferative stage. (A) Cell-cycle analysis of WT and p15+ retinae by flow cytometry. The first row is the WT group (G1 to G2 ratio is 2.8), and the second row is the p15+ group (G1 to G2 ratio is 4.6). (B) Representative images showing neurogenesis wave in WT and p15+ retinae by SoFa fish line, in which RGCs are labeled by Tg(atoh7:gapRFP), ACs and HCs are labeled by Tg(ptf1α:GFP), and PRs and BCs were labeled by Tg(crx:CFPcaax). Areas within white dashed contour lines are retinae. (C) F-actin distribution visualized by GFP-Utrch mRNA injection in control (top) and p15 overexpression (bottom) retinae. Zoom-in images of retinal areas (dashed yellow rectangles) are shown in the right panels. Areas within white dashed contour lines are retinae. (D) Representative images showing the nuclei of all cell types labeled in blue by Tg(actin:H2BCFP) and ACs labeled in magenta by Tg(ptf1α:DsRed). Yellow arrowheads indicate an AC (blue and magenta double positive, n = 69) and a BC (blue single positive, n = 80). (E) Quantification of nuclear diameters of BCs and ACs. (F and G) Representative cases (F) and quantification of cell-cycle length (G) of single 12-hpf WT donor RPCs in WT (top, n = 30) and p15+ (bottom, n = 10) host retinae. Yellow arrowheads show RPCs at the M phase. (H and I) Representative images (H) and quantification of size (I) of WT donor clones derived from single 12-hpf RPCs in scramble-sg (n = 25) and slbp1-disrupted (n = 7) host retinae. (J and K) Representative images (J) and quantification of size (K) of WT donor clones derived from 12-hpf RPCs upon atoh7 expression indicated by Tg(atoh7:GFP) in WT host retinae (left, n = 28) and p15+ host retinae (right, n = 16). Inserts in J describe the schematic of transplantation. The white circles indicate the first cell to turn on atoh7-GFP in one clone. (L and M) Representative images (L) and quantification of clone size (M) of 72-hpf WT donor isolated atoh7+ clones in WT host retinae (left, n = 65) and p15+ host retinae (right, n = 38). The dashed white lines represent the OPL (up) and the IPL (down) of the retinae in L. Data in E, G, I, K, and M represent mean ± SEM. Scale bars, 10 µm. OPL, the outer plexiform layer; IPL, the inner plexiform layer.

p15 + hosts induce faster proliferation of WT donor RPCs at the early proliferative stage. (A) Cell-cycle analysis of WT and p15+ retinae by flow cytometry. The first row is the WT group (G1 to G2 ratio is 2.8), and the second row is the p15+ group (G1 to G2 ratio is 4.6). (B) Representative images showing neurogenesis wave in WT and p15+ retinae by SoFa fish line, in which RGCs are labeled by Tg(atoh7:gapRFP), ACs and HCs are labeled by Tg(ptf1α:GFP), and PRs and BCs were labeled by Tg(crx:CFPcaax). Areas within white dashed contour lines are retinae. (C) F-actin distribution visualized by GFP-Utrch mRNA injection in control (top) and p15 overexpression (bottom) retinae. Zoom-in images of retinal areas (dashed yellow rectangles) are shown in the right panels. Areas within white dashed contour lines are retinae. (D) Representative images showing the nuclei of all cell types labeled in blue by Tg(actin:H2BCFP) and ACs labeled in magenta by Tg(ptf1α:DsRed). Yellow arrowheads indicate an AC (blue and magenta double positive, n = 69) and a BC (blue single positive, n = 80). (E) Quantification of nuclear diameters of BCs and ACs. (F and G) Representative cases (F) and quantification of cell-cycle length (G) of single 12-hpf WT donor RPCs in WT (top, n = 30) and p15+ (bottom, n = 10) host retinae. Yellow arrowheads show RPCs at the M phase. (H and I) Representative images (H) and quantification of size (I) of WT donor clones derived from single 12-hpf RPCs in scramble-sg (n = 25) and slbp1-disrupted (n = 7) host retinae. (J and K) Representative images (J) and quantification of size (K) of WT donor clones derived from 12-hpf RPCs upon atoh7 expression indicated by Tg(atoh7:GFP) in WT host retinae (left, n = 28) and p15+ host retinae (right, n = 16). Inserts in J describe the schematic of transplantation. The white circles indicate the first cell to turn on atoh7-GFP in one clone. (L and M) Representative images (L) and quantification of clone size (M) of 72-hpf WT donor isolated atoh7+ clones in WT host retinae (left, n = 65) and p15+ host retinae (right, n = 38). The dashed white lines represent the OPL (up) and the IPL (down) of the retinae in L. Data in E, G, I, K, and M represent mean ± SEM. Scale bars, 10 µm. OPL, the outer plexiform layer; IPL, the inner plexiform layer.

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