Macrophage Nrf2 boosts homeostatic patrolling and pro-inflammatory behavior. (A and B) Average speed and directionality of laterally migrating stage 13 control and srp > Nrf2^RNAi macrophages. (C and D) Average speed and CIL behavior of stage 15 control and srp > Nrf2^RNAi macrophages. (E–G) Nrf2 knockdown (srp > Nrf2^RNAi) increased the number of phagosomes per macrophage and caused a modest increase in cell body size (hypertrophy). (H and I) Wound recruitment defect upon macrophage-specific Nrf2^RNAi analyzed at stage 15 of embryonic development. (J) Macrophage Nrf2^RNAi decreased the migrational bias toward sites of epithelial damage, as measured by the angle α relative to the center of the wound. (K) 18 h APF pupal macrophages strongly upregulated Nrf2 upon wounding. Cell bodies (E), site of epithelial wounds (H), and insets (K) are indicated by white dashed outlines. Macrophages labeled in green (srp > GFP, E, H) or magenta (srp-mcherry, K). Macrophage nuclei labeled in red (srp > H2A::mcherry, H), Nrf2 in green (Nrf2-GFP, K). All Nrf2^RNAi data were generated using Nrf2^RNAi Flybase ID FBtp0069370. PW: post-wounding. ns: not significant, *, P < 0.05; **P < 0.01, ****P < 0.0001 via Mann-Whitney test (A, B, C, F, G, J) or unpaired t-test (I), one-way ANOVA followed by Dunn’s comparison analysis (K). Images were collected from 5 control and 5 srp > Nrf2^RNAi embryos (A, B); 5 control and 5 srp > Nrf2^RNAi embryos (C); stage 13: 5 control and 12 srp > Nrf2^RNAi embryos and stage 15: 24 control and 33 srp > Nrf2^RNAi embryos (F); 44 control, 58 srp > Nrf2^RNAi embryos (G); 7 control and 7 srp > Nrf2^RNAi embryos (I, I′); 4 pupae (K).