Role of Aurora B in anaphase error correction and the spatiotemporal coordination of mitotic exit. The midzone-based Aurora B activity gradient was proposed to establish a chromosome separation checkpoint that delays chromosome decondensation and NER on incompletely separated chromosomes, including laggards. This allows their correction and reintegration into the main nuclei (Aurora B activity gradient ON). By inhibiting Aurora B activity or preventing its midzone localization, NER is initiated synchronously on all chromosomes, regardless of separation (Aurora B activity gradient OFF), independently of the presence of midzone/midbody microtubule bundles. White arrowheads indicate lagging chromosomes. Time-lapse series was modified from Orr et al. (2021). Scale bar is 5 μm. MT, microtubule.