mRNA regulates the recruitment of EPG-2 to PGL granules, related to Fig. 7 . (A) Sedimentation assays showing that mRNA reduces the level of GFP::EPG-2 that partitions into condensates. (B) The direct interaction between SEPA-1 and EPG-2 is not evidently affected by RNase A treatment or the addition of 30 ng/μl mRNA in the reactions in the in vitro GST pull-down assay. Asterisks indicate bands with the expected molecular mass. The level of EPG-2 pulled down by GST::SEPA-1 was set to 1.0 in control conditions. (C and D) Compared with control embryos shown in (Fig. 7 D), the colocalization ratio of EPG-2 aggregates and PGL granules, detected by anti-SEPA-1, is increased in snr-4(RNAi) (C) and prp-19(RNAi) (D) embryos at 26°C. Embryos at the ∼100-cell stage are shown in C and D. (E and F) Compared to epg-2 mutant embryos shown in (Fig. 7 J), the number of PGL granules, detected by PGL-3 antibody, is reduced by simultaneous snr-4(RNAi) (E) and pab-1(RNAi) (F) at 26°C. Maximum-intensity projections of Z-stack confocal images of comma-stage embryos are shown in E and F. Scale bars: 5 µm for C–F; 2 µm for enlarged images in C and D. Source data are available for this figure: SourceData FS5.