Figure 5.
Loss of function of factors involved in mRNA turnover restores PGL granule accumulation in sept-6 mutants. (A) Summary of identified RNAi inactivations that restore PGL granule accumulation in sept-6 mutants under heat stress conditions. (B–E) Compared with control(RNAi)-treated sept-6(tm6608) mutant embryos at 26°C (B), many more PGL granules, labeled by GFP::SEPA-1, are formed in sept-6 mutant embryos treated with ntl-11(RNAi) (C), xrn-1(RNAi) (D), and eri-6(RNAi) (E). (F) Quantification of the number of PGL granules per embryo in the indicated genetic backgrounds at 26°C. Data are shown as mean ± SEM (n = 3; n refers to the number of embryos analyzed for each genotype). Two-tailed, unpaired t test results: *P < 0.05, **P < 0.01, ***P < 0.001. (G–I) Compared to sept-6(tm6608) mutant embryos (G), many more PGL granules, detected by anti-PGL-3, are formed in dcr-1(bp132); sept-6(tm6608) mutant embryos (H) at 26°C. I shows the quantitative data (mean ± SEM, n = 3; n refers to the number of embryos analyzed for each genotype). Two-tailed, unpaired t test result: **P < 0.01. Maximum-intensity projections of Z-stack confocal images of comma-stage embryos are shown in B–E, G, and H. (J) The protein level of SEPA-1 is increased in extracts of dcr-1(bp132); sept-6(tm6608) mutant embryos compared with sept-6(tm6608) embryos at 26°C. Levels of SEPA-1 are normalized with the corresponding ACTIN level. Scale bars: 5 µm for B–E, G, and H. Source data are available for this figure: SourceData F5.

Loss of function of factors involved in mRNA turnover restores PGL granule accumulation in sept-6 mutants. (A) Summary of identified RNAi inactivations that restore PGL granule accumulation in sept-6 mutants under heat stress conditions. (B–E) Compared with control(RNAi)-treated sept-6(tm6608) mutant embryos at 26°C (B), many more PGL granules, labeled by GFP::SEPA-1, are formed in sept-6 mutant embryos treated with ntl-11(RNAi) (C), xrn-1(RNAi) (D), and eri-6(RNAi) (E). (F) Quantification of the number of PGL granules per embryo in the indicated genetic backgrounds at 26°C. Data are shown as mean ± SEM (n = 3; n refers to the number of embryos analyzed for each genotype). Two-tailed, unpaired t test results: *P < 0.05, **P < 0.01, ***P < 0.001. (G–I) Compared to sept-6(tm6608) mutant embryos (G), many more PGL granules, detected by anti-PGL-3, are formed in dcr-1(bp132); sept-6(tm6608) mutant embryos (H) at 26°C. I shows the quantitative data (mean ± SEM, n = 3; n refers to the number of embryos analyzed for each genotype). Two-tailed, unpaired t test result: **P < 0.01. Maximum-intensity projections of Z-stack confocal images of comma-stage embryos are shown in B–E, G, and H. (J) The protein level of SEPA-1 is increased in extracts of dcr-1(bp132); sept-6(tm6608) mutant embryos compared with sept-6(tm6608) embryos at 26°C. Levels of SEPA-1 are normalized with the corresponding ACTIN level. Scale bars: 5 µm for B–E, G, and H. Source data are available for this figure: SourceData F5.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal