Figure 3.
Loss of function of factors involved in mRNA metabolism suppresses the accumulation of PGL granules in embryos laid under heat stress conditions. (A) Summary of RNAi inactivations that suppress PGL granule accumulation in embryos under heat stress. (B–E) Compared to control(RNAi) (B), the number of PGL granules, labeled by GFP::PGL-3, is decreased in snr-4(RNAi) (C), ruvb-1(RNAi) (D), and pab-1(RNAi) (E) embryos at 26°C. Maximum-intensity projections of Z-stack confocal images of comma-stage embryos are shown in B–E. (F and G) Quantification of PGL granules per embryo in the indicated genetic backgrounds at 26°C. Data are shown as mean ± SEM (n = 3; n refers to the number of embryos analyzed for each genotype). Two-tailed, unpaired t test results: n.s.: no significant difference, **P < 0.01, ***P < 0.001. Scale bars: 5 µm for B–E.

Loss of function of factors involved in mRNA metabolism suppresses the accumulation of PGL granules in embryos laid under heat stress conditions. (A) Summary of RNAi inactivations that suppress PGL granule accumulation in embryos under heat stress. (B–E) Compared to control(RNAi) (B), the number of PGL granules, labeled by GFP::PGL-3, is decreased in snr-4(RNAi) (C), ruvb-1(RNAi) (D), and pab-1(RNAi) (E) embryos at 26°C. Maximum-intensity projections of Z-stack confocal images of comma-stage embryos are shown in B–E. (F and G) Quantification of PGL granules per embryo in the indicated genetic backgrounds at 26°C. Data are shown as mean ± SEM (n = 3; n refers to the number of embryos analyzed for each genotype). Two-tailed, unpaired t test results: n.s.: no significant difference, **P < 0.01, ***P < 0.001. Scale bars: 5 µm for B–E.

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