Figure S3.
Endogenous TNIP1 interacts with TAX1BP1 and p62/SQSTM1 under basal conditions. (A) SILAC-based, IP-MS analyses of anti-TNIP1 immunoprecipitations identified TAX1BP1 and p62/SQSTM1 as enriched compared to negative control IPs using beads only. GAPDH is shown as negative control. Shown are average values of three biological replicates (n = 3). Error bars: SD, ** = P < 0.01, *** = P < 0.001, unpaired, two-sided t test. (B and C) Lysosomal turnover of TNIP1 is mediated by several SLRs. HeLa WT and HeLa p62, OPTN, and TAX1BP1 KO cells were either left untreated or treated with 200 nM BafA1 for 12 h. Shown are average values of three biological replicates. Error bars: SD, * = P < 0.05, unpaired, two-sided t test. Source data are available for this figure: SourceData FS3.

Endogenous TNIP1 interacts with TAX1BP1 and p62/SQSTM1 under basal conditions. (A) SILAC-based, IP-MS analyses of anti-TNIP1 immunoprecipitations identified TAX1BP1 and p62/SQSTM1 as enriched compared to negative control IPs using beads only. GAPDH is shown as negative control. Shown are average values of three biological replicates (n = 3). Error bars: SD, ** = P < 0.01, *** = P < 0.001, unpaired, two-sided t test. (B and C) Lysosomal turnover of TNIP1 is mediated by several SLRs. HeLa WT and HeLa p62, OPTN, and TAX1BP1 KO cells were either left untreated or treated with 200 nM BafA1 for 12 h. Shown are average values of three biological replicates. Error bars: SD, * = P < 0.05, unpaired, two-sided t test. Source data are available for this figure: SourceData FS3.

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