TNIP1 localizes to autophagosomes. U2OS cells kept in either fed or starved (HBSS) conditions and treated with either vehicle (DMSO) or BafA1 for 8 h. Cells were fixed and stained with antibodies against endogenous TNIP1 (green), p62 (orange), and LC3 (purple) and imaged using the Zeiss LSM800 confocal microscope. Line-profile co-localization plots were made using the line-profile quantification tool in the Zen blue imaging software (Zeiss). Vertical axis represents measurements of fluorescent intensity and the horizontal axis the drawn distances. Scale bar = 10 µm.