scRNA-seq of hashtags for PBS and TLR ligand stimulated m ononuclear phagocytes in the LLN. (A) UMAP demonstrating the distribution of all cells acquired on the 10X from the five different TLR adjuvant treatment groups: no treatment control (Control), CpG, LPS, Poly-IC, R848. Dot chart shows curated genes and the top 10 DEGs for the entire dataset. (B) Re-clustering identified mononuclear phagocytes, UMAP demonstrating the distribution of cells from the five different TLR adjuvant treatment groups: no treatment control (Control), CpG, LPS, Poly-IC, R848. Dot chart shows the top 10 DEGs in samples with each treatment group compared with no treatment control for the seven specific myeloid cell types. UMAP annotations and platform for self-analysis: https://cells.ucsc.edu/?ds=ln-mono-dc. (C) Dot chart shows mregDC marker genes across defined clusters. MC, mast cells; Mono, monocytes; Cyc.Mye, cycling myeloid cells; Cyc.Lym., cyclin lymphocytes; FB, fibroblasts; NK, natural killer; pDC, plasmacytoid DC.