Visual rescue determined by visually driven optomotor behavior test in the Pde6b ^rd10 mice treated with PE ^SpRY system and gPde6b. (A) The automated optomotor system setup. (B) Visual function is evaluated through a series of stimuli at defined spatial frequencies and contrasts. (C and D) The equal contribution of both left and right eyes in the wild-type mice to the optomotor responses at defined spatial frequencies (C) or contrasts (D). Representative results were shown, and data are presented as mean ± SEM. (E and F) The visual stimuli–driven optomotor responses are globally significant in the Pde6b^{T to C} context when tested at defined spatial frequencies (E) or contrasts (F). Representative heat maps indicate OMR values at a wide range of velocity thresholds at defined spatial frequency or contrast levels. (G and H) The visual stimuli–driven optomotor responses are significant in the Pde6b^{T to C} context when tested at defined spatial frequencies (G) or contrasts (H) under the optimal velocity conditions (2–14°/s). The response time in either stimulus direction (positive value, light green window) or opposite direction (negative value, light magenta window) at defined velocity thresholds is normalized to the maximal response time, which is herein defined as 1 and presented as a blue bar. The OMR values at each spatial frequency or contrast are indicated with a magenta bar normalized to green one defined as 1 if the response time in the stimulus direction is longer than that in the opposite direction, and vice versa. Here, the heights of green bars are the same as those of light green bars when the response time in the stimulus direction is longer than that in the opposite direction, so the light green bars are covered by green bars. Representative results were shown. (I and J) The difference between the head moving time in the stimulus direction and that in the opposite direction, namely ΔT, in the Pde6b^{T to C} context intuitively shows a parallel change with the situation in the wild-type mice when tested at defined spatial frequencies (I) or contrasts (J) under the optimal velocity conditions (2–14°/s). Representative data are presented as mean ± SEM; n ≥ 8 independent biological replicates. Two-way ANOVA tests with Tukey’s multiple comparisons. **, P < 0.01; ***, P < 0.001. All of Pde6b^rd10 control mice were treated with PE^SpRY and gScrambled. All experiments were independently performed at least three times to ensure repeatable results.