Transcriptomic analysis assessing the effect of cMyBP-C ablation in hiPSC-ECT. (A–D) Transcriptomic analysis of all genes expressed in hiPSC-ECTs (A), sarcomeric protein encoding and hypertrophic marker genes (B), genes involved in myocardial Ca²⁺ handling and signaling (C), and genes involved in glucose metabolism and fatty acid oxidation (D). In A–D, the leftmost panels show log-fold change vs. −log FDR volcano plots of cMyBP-C^+/+ vs. cMyBP-C^+/−; the center panels show log-fold change vs. −log FDR volcano plots of cMyBP-C^+/+ vs. cMyBP-C^−/−. In A, the rightmost plot shows a Venn diagram comparing gene expression in cMyBP-C^+/+, cMyBP-C^+/−, and cMyBP-C^−/− hiPSC-ECTs, while in B–D they show heat maps comparing gene expression in cMyBP-C^+/+, cMyBP-C^+/−, and cMyBP-C^−/− hiPSC-ECTs of genes in the respective GO pathways. In volcano plots, differentially expressed genes (FDR < 0.05) are plotted as red dots. In heat maps, red indicates upregulated genes, while blue indicates genes that are downregulated. Z-scores > 15 are highlighted in bright red.