Effect of cMyBP-C ablation on myofilament Ca2+sensitivity in hiPSC-ECT. (A and B) Averaged absolute (A) and normalized (B) pCa vs. force traces in detergent permeabilized cMyBP-C+/+ (broken traces), cMyBP-C+/− (grey traces), and cMyBP-C−/− (black traces) hiPSC-ECTs. (C–H) Box and whisker plots of maximum Ca2+-activated force produced (C), pCa50 (D), and Hill coefficient (E), force at pCa 5.8 expressed as a fraction of maximum Ca2+-activated force (F), and the maximum velocities of force regeneration at pCa 5.8 (G) and pCa 4.5 (H). All experiments were performed at 37°C. n ≥ 11 biological replicates; *P < 0.05; **P < 0.01; ***P < 0.001 as calculated by one-way ANOVA with Tukey’s multiple comparisons test; see Table S8 for specific P values.
Figure 6.

Effect of cMyBP-C ablation on myofilament Ca 2+ sensitivity in hiPSC-ECT. (A and B) Averaged absolute (A) and normalized (B) pCa vs. force traces in detergent permeabilized cMyBP-C+/+ (broken traces), cMyBP-C+/− (grey traces), and cMyBP-C−/− (black traces) hiPSC-ECTs. (C–H) Box and whisker plots of maximum Ca2+-activated force produced (C), pCa50 (D), and Hill coefficient (E), force at pCa 5.8 expressed as a fraction of maximum Ca2+-activated force (F), and the maximum velocities of force regeneration at pCa 5.8 (G) and pCa 4.5 (H). All experiments were performed at 37°C. n ≥ 11 biological replicates; *P < 0.05; **P < 0.01; ***P < 0.001 as calculated by one-way ANOVA with Tukey’s multiple comparisons test; see Table S8 for specific P values.

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