Effect of cMyBP-C ablation on contractile function in hiPSC-ECT. (A and B) Absolute (A) and normalized (B) averaged twitch force traces of 40–54 d old cMyBP-C+/+ (broken traces), cMyBP-C+/− (grey traces), and cMyBP-C−/− (black traces) hiPSC-ECTs. (C and D) Normalized average traces focusing on the time from peak to 50% (RT50; C) and from 50 to 90% twitch force decay (RT50-90; D). (E–H) Box and whisker plots of maximum twitch force amplitude (TF; E); time to peak twitch force (CT100; F); time from peak to 50% twitch force decay (RT50; G) and time from 50 to 90% twitch force decay (RT50-90; H). All experiments were performed at 37°C with pacing at 1.5 Hz. n ≥ 17 biological replicates; *P < 0.05; ***P < 0.001 as calculated by one-way ANOVA with Tukey’s multiple comparisons test; see Table S6 for specific P values.
Figure 3.

Effect of cMyBP-C ablation on contractile function in hiPSC-ECT. (A and B) Absolute (A) and normalized (B) averaged twitch force traces of 40–54 d old cMyBP-C+/+ (broken traces), cMyBP-C+/− (grey traces), and cMyBP-C−/− (black traces) hiPSC-ECTs. (C and D) Normalized average traces focusing on the time from peak to 50% (RT50; C) and from 50 to 90% twitch force decay (RT50-90; D). (E–H) Box and whisker plots of maximum twitch force amplitude (TF; E); time to peak twitch force (CT100; F); time from peak to 50% twitch force decay (RT50; G) and time from 50 to 90% twitch force decay (RT50-90; H). All experiments were performed at 37°C with pacing at 1.5 Hz. n ≥ 17 biological replicates; *P < 0.05; ***P < 0.001 as calculated by one-way ANOVA with Tukey’s multiple comparisons test; see Table S6 for specific P values.

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