CRISPR-Cas9 genome editing of exons 2 and 6 of MYBPC3.(A) sgRNA + Cas9-2A-PAC plasmid map. (B) Timeline of genome editing and selection of DF19-9-11T.H hiPSC cells. (C) Schematic representation of Cas9-mediated gene editing by NHEJ. (D) Schematic of droplet digital PCR design. (E) Schematic of droplet digital PCR results in a non-targeted (left), heterozygously targeted (center), and homozygously targeted (right) iPSC colony. (F) Partial DNA sequence of exon 6 of MYBPC3 of a wild type allele (top) and the 17 bp deletion allele in clone CR1F2, heterozygous for a cMyBP-C truncation mutation. (G) Partial DNA sequence of exon 2 of MYBPC3 of a 1-bp deletion allele (top) and a 5-bp deletion allele in clone CR8 12-1, homozygous for a cMyBP-C truncation mutations.
Figure S1.

CRISPR-Cas9 genome editing of exons 2 and 6 of MYBPC3. (A) sgRNA + Cas9-2A-PAC plasmid map. (B) Timeline of genome editing and selection of DF19-9-11T.H hiPSC cells. (C) Schematic representation of Cas9-mediated gene editing by NHEJ. (D) Schematic of droplet digital PCR design. (E) Schematic of droplet digital PCR results in a non-targeted (left), heterozygously targeted (center), and homozygously targeted (right) iPSC colony. (F) Partial DNA sequence of exon 6 of MYBPC3 of a wild type allele (top) and the 17 bp deletion allele in clone CR1F2, heterozygous for a cMyBP-C truncation mutation. (G) Partial DNA sequence of exon 2 of MYBPC3 of a 1-bp deletion allele (top) and a 5-bp deletion allele in clone CR8 12-1, homozygous for a cMyBP-C truncation mutations.

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