Cardiomyopathy mutations occur in regions involved in ADP release. (A) Cartoon diagram of the nucleotide-free β-cardiac myosin II S1 domain highlighting the α-carbon of residues that are known cardiomyopathy-causing mutations. Spheres indicate loci for mutations leading to hypertrophic cardiomyopathy (red), dilated cardiomyopathy (green), or both HCM and DCM (yellow). Each residue was found using the ClinVar archive filtering residues that are known to be pathogenic. (B) Shown is the location of myosin residue F764, which sits at the interface between the converter domain and the relay helix. The mutation F764 L is known to lead to DCM. (C) Shown is the location of the residue R712, which also sits at the interface between the converter domain and the relay helix. Mutations at this site are known to cause HCM. A cluster of mutations are fund in the converter domain, making it a hotspot of mutations.