Local resolution and map quality. (A) The FSC curve and local resolution estimations calculated in RELION for the indicated helical reconstructions. These plots show that despite the high global resolution (as measured with the FSC curve), the local resolution of the reconstruction varies. Both reconstructions contain high resolutions toward the interior of the maps, while the resolution decreases radially from the actin axis. Below these are map-to-model comparisons for the helical reconstruction in the actin and myosin regions. (B) FSC curves and local resolution estimations calculated in RELION for the indicated single-particle reconstructions. From these plots, it is observed that the local resolution of the single-particle reconstructions does not deviate as much as the helical reconstructions. These focused refinements allowed us to visualize the light chain at the distal regions of the myosin motor. Below these are map-to-model comparisons of the indicated myosin region.