The Panx1 inhibitory peptide ¹⁰ p anx1 and the P2Y2 antagonist AR-C118925 do not affect voltage-activated SR Ca ²⁺ release. (A) Voltage dependence of the peak amplitude of SR Ca²⁺ release in fibers treated with 200 µM of either the ¹⁰panx1 peptide (red) or with the scrambled control peptide (¹⁰panx1SCr, black). Individual datapoints from each muscle fiber are shown, with the corresponding Boltzmann fit superimposed (continuous line). The ¹⁰panx1SCr and ¹⁰panx1 datasets are from six fibers and four fibers from two mice, respectively. Data from muscle fibers issued from the same mouse are shown with the same symbol. The inset on the right shows the corresponding mean (±SD) values in each group with the x and y scale covering the same ranges as in the main panel. Individual and mean (±SD) values for the Boltzmann parameters in the two groups of muscle fibers are shown in the bar graphs. The absence of statistical difference between the parameters in the two groups was assessed using a nested analysis (Eisner, 2021). (B) Voltage dependence of the peak amplitude of SR Ca²⁺ release in control fibers (black) and in fibers treated with 10 µM of AR-C118925 (red). Individual datapoints from each muscle fiber are shown, with the corresponding Boltzmann fit superimposed (continuous line). The control and AR-C118925 datasets are from nine fibers and nine fibers from three mice. Data presentation and analysis are as described in A.