NFAT2 is required in CD4 ⁺ T cells for development of murine lupus. (A) Photograph of spleens isolated from aged SLE or NFAT2 cKO-SLE mice. Scale bar = 1 cm. (B) Numbers of splenic immune cell populations measured by flow cytometry comparing age-matched B6, SLE, and cKO-SLE mice. (C) Numbers of splenic T cell subsets. (D) Numbers of subtypes of splenic B cells. (E) Percentage of IgG1⁺ or IgG2c⁺ positive plasmablasts. (F) Serum dsDNA autoantibody titers from B6, SLE, or cKO-SLE mice measured by ELISA. T and B cell subsets are gated as described in Fig. 6. Results representative of three independent experiments with two to five animals per group. One-way ANOVA was used to analyze the data. ** = P ≤ 0.01, * = P ≤ 0.05, and ns P > 0.05.