Figure S3.
The impact of infection on intestinal CD103+TRMcell differentiation and the residency of intestinal memory CD8 T cells derived from spleen-primed T cells. (A) Experimental design for B and C. 1 × 104 naive OT-I cells were transferred into congenic naive first recipient mice. 1 d later, mice were foodborne infected with InlAMLm-OVA. At 5.5 dpi, effector OT-I cells were isolated from the MLN and transferred into congenic second recipient mice that were foodborne infected with InlAMLm-OVA or treated with sham PBS 5.5 d previously. 3 d after transfer, donor OT-I cells in second recipient mice were analyzed. (B) The expression of CD69 and CD103 by donor OT-I cells in the LP and IEL compartment of indicated second recipient mice. (C) Numbers of donor OT-I cells in tissues of indicated second recipient mice. (D) The expression of CD127 and KLRG-1 by donor OT-I cells in indicated tissues at >30 d after transfer into congenic naive second recipient mice. (E) Experimental design for F. 1 × 104 naive OT-I cells were transferred into congenic naive first recipient mice 1 d prior to i.v. infection with InlAMLm-OVA. At 5.5 dpi, effector OT-I cells were isolated from the spleen and transferred into congenic second recipient mice that were uninfected or foodborne infected with InlAMLm-OVA 5.5 d previously. 3 d after transfer, donor OT-I cells in second recipient mice were analyzed. (F) The expression of CD69 and CD103 by donor OT-I cells in the IEL compartment of indicated second recipient mice. (G) Experimental design for H. 1 × 104 naive OT-I cells were transferred into congenic naive first recipient mice 1 d prior to i.v. infection with InlAMLm-OVA. At 5.5 dpi, effector OT-I cells were isolated from the spleen and transferred into congenic naive second recipient mice. 6 d after transfer, second recipient mice were treated with vehicle control or FTY720 daily for 3 wk followed by analysis of donor OT-I cells. (H) The expression of CD69 and CD103 by donor OT-I cells in the LP and IEL compartment of indicated second recipient mice. The data in B and C are pooled from two independent experiments, n = 8–9 mice total. The data in D are representative of two independent experiments, n = 4 mice/experiment. The data in F are pooled from two independent experiments, n = 3–4 mice total. The data in H are pooled from two independent experiments, n = 5–6 mice total. The data are expressed as mean ± SEM. Unpaired t tests were performed (B–D, F and H). *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001.

The impact of infection on intestinal CD103 + T RM cell differentiation and the residency of intestinal memory CD8 T cells derived from spleen-primed T cells. (A) Experimental design for B and C. 1 × 104 naive OT-I cells were transferred into congenic naive first recipient mice. 1 d later, mice were foodborne infected with InlAMLm-OVA. At 5.5 dpi, effector OT-I cells were isolated from the MLN and transferred into congenic second recipient mice that were foodborne infected with InlAMLm-OVA or treated with sham PBS 5.5 d previously. 3 d after transfer, donor OT-I cells in second recipient mice were analyzed. (B) The expression of CD69 and CD103 by donor OT-I cells in the LP and IEL compartment of indicated second recipient mice. (C) Numbers of donor OT-I cells in tissues of indicated second recipient mice. (D) The expression of CD127 and KLRG-1 by donor OT-I cells in indicated tissues at >30 d after transfer into congenic naive second recipient mice. (E) Experimental design for F. 1 × 104 naive OT-I cells were transferred into congenic naive first recipient mice 1 d prior to i.v. infection with InlAMLm-OVA. At 5.5 dpi, effector OT-I cells were isolated from the spleen and transferred into congenic second recipient mice that were uninfected or foodborne infected with InlAMLm-OVA 5.5 d previously. 3 d after transfer, donor OT-I cells in second recipient mice were analyzed. (F) The expression of CD69 and CD103 by donor OT-I cells in the IEL compartment of indicated second recipient mice. (G) Experimental design for H. 1 × 104 naive OT-I cells were transferred into congenic naive first recipient mice 1 d prior to i.v. infection with InlAMLm-OVA. At 5.5 dpi, effector OT-I cells were isolated from the spleen and transferred into congenic naive second recipient mice. 6 d after transfer, second recipient mice were treated with vehicle control or FTY720 daily for 3 wk followed by analysis of donor OT-I cells. (H) The expression of CD69 and CD103 by donor OT-I cells in the LP and IEL compartment of indicated second recipient mice. The data in B and C are pooled from two independent experiments, n = 8–9 mice total. The data in D are representative of two independent experiments, n = 4 mice/experiment. The data in F are pooled from two independent experiments, n = 3–4 mice total. The data in H are pooled from two independent experiments, n = 5–6 mice total. The data are expressed as mean ± SEM. Unpaired t tests were performed (B–D, F and H). *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001.

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