Figure 6.
Cartoon of pre-60S assembly. Numbers 1–3 represent three potential QC steps: (1) inspecting A-site methylation, (2) testing PTC assembly, and (3) testing translational GTPase activation. Described in more detail in the section titled “Overview of pre-60S assembly.” The 5S RNP (consisting of the 5S rRNA, Rpl5, and Rpl11) is green and 25S:Gm2922 methylation is represented by a red star. SAM: S-adenosyl-methionine; SAH: S-adenosyl-homocysteine.