Figure 2.
Human atlastins can replace Sey1p to support ER microsome fusion. (A) ER microsomes were isolated from the BJ-Gluc1 sey1Δ and BJ-Gluc2 sey1Δ yeast strains expressing ATL1, ATL2, or ATL3, and incubated on ice or at 27°C in the presence of GTP or GTPγS. After 90 min, luciferase activity was measured as previously described (Lee et al., 2015). Data represent the means ± SEM (error bars; n = 3). n refers to the number of independent experiments. (B) Protein profiles of ER microsomes used in the experiments are shown in A. Expression of Gluc protein fragment complementation assay fragments and myc-tagged human atlastins was analyzed by immunoblotting using the indicated antibodies. The ER-resident protein Yet3p was used as a loading control. Source data are available for this figure: SourceData F2.

Human atlastins can replace Sey1p to support ER microsome fusion. (A) ER microsomes were isolated from the BJ-Gluc1 sey1Δ and BJ-Gluc2 sey1Δ yeast strains expressing ATL1, ATL2, or ATL3, and incubated on ice or at 27°C in the presence of GTP or GTPγS. After 90 min, luciferase activity was measured as previously described (Lee et al., 2015). Data represent the means ± SEM (error bars; n = 3). n refers to the number of independent experiments. (B) Protein profiles of ER microsomes used in the experiments are shown in A. Expression of Gluc protein fragment complementation assay fragments and myc-tagged human atlastins was analyzed by immunoblotting using the indicated antibodies. The ER-resident protein Yet3p was used as a loading control. Source data are available for this figure: SourceData F2.

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