Differential effects of cholesterol and PE on atlastin-mediated liposome fusion. (A–C) Liposome fusion was assayed as in Fig. 1 A using ATL1-liposomes (A), ATL2-liposomes (B), or ATL3-liposomes (C) bearing different concentrations of cholesterol and PE. Donor and acceptor proteoliposomes were mixed and incubated in the absence or presence of GTP at 30°C for 40 min. Fusion is expressed as the percentage of total NBD fluorescence. Data represent the means ± SEM (error bars; n = 3). n refers to the number of independent experiments. *P < 0.05, one-way ANOVA with Tukey’s multiple comparisons test.
Figure S2.

Differential effects of cholesterol and PE on atlastin-mediated liposome fusion. (A–C) Liposome fusion was assayed as in Fig. 1 A using ATL1-liposomes (A), ATL2-liposomes (B), or ATL3-liposomes (C) bearing different concentrations of cholesterol and PE. Donor and acceptor proteoliposomes were mixed and incubated in the absence or presence of GTP at 30°C for 40 min. Fusion is expressed as the percentage of total NBD fluorescence. Data represent the means ± SEM (error bars; n = 3). n refers to the number of independent experiments. *P < 0.05, one-way ANOVA with Tukey’s multiple comparisons test.

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