TBMs in GCs are replaceable. (A) Mice were treated with PBS or DT for every second day for 5 d, followed by 7 additional recovery days. Representative flow cytometry plots showing CX3CR1⁺ cells in PPs. (B) Immunofluorescence staining and quantification of PP CD68⁺ macrophages in GCs. Each dot represents an individual GC; three to four mice per condition in three experiments. (C) GC B cells in PBS-treated and DT-treated mice; three to four mice per condition in three experiments. Each dot represents a mouse. (D) Immunofluorescence staining of inguinal LNs derived from PBS or DT-treated mice at the indicated time points after the initial immunization. Each dot represents a GC. Data were collected from three independent experiments; three to four mice were used in each experiment. ***, P < 0.001; ns, not significant; one-way ANOVA.