Subcapsular macrophages do not give rise to TBMs. (A) Scheme showing the experimental layout. (B) Representative flow cytometry plots showing CD169⁺ cells in inguinal LNs 7 d after NP-KLH immunization. Each dot represents a single mouse. n = 5 mice; two independent experiments. (C and D) Immunofluorescence staining of LNs derived from PBS- and DT-treated mice. (E) Quantification CD68⁺ cell density in GCs. Each dot represents a ratio in a single GC. n = 5 mice, two independent experiments. Line represents the mean. ***, P < 0.001; ns, not significant; two-tailed Student’s t test.