Tetrad analysis of cross between sec2-41 and strains containing integrated forms of EXO84 gain-of-function alleles. Yeast strain (BY3263: α, sec2-41, his3-200) was crossed to three strains containing integrated copies of EX084 marked with nourseothricin (Nat) resistance: BY3311: a, EXO84::NatR, ura3-52, leu2-3,112; BY3312: a, EXO84-L365W::NatR, ura3-52, leu2-3,112; and BY3313: EXO84-E626V::NatR; ura3-52, leu2-3,112. Following sporulation tetrads were dissected, grown at 25C for 2 d and then replica-plated onto various media and grown at temperatures as indicated in the figure.