Figure 3.
Sro7-NT peptide stimulates exocyst binding to Sec4-GTP but not Snc2. (A) Purified wild-type exocyst binding to immobilized GST-Sec4 beads exchanged with either GTPγS or control GDP in the presence of wild-type Sro7-NT (aa 1–90) peptide or control mutant Sro7-NT-A5,A6. Quantitation of two separate subunits one from each sub-complex is shown to the right. Error bars represent SD. P values were obtained using a two-tailed Student’s t test. *, P <0.05; **, P <0.01; *** P <0.001; ns = no significant difference. (B) Purified exocyst complex binding to immobilized GST-Snc2 or control GST-Snc2E79,E82 beads in the presence of wild-type Sro7-NT (aa 1–90) peptide or control mutant Sro7-NT-A5,A6 peptide. Quantitation of two separate subunits, one from each exocyst sub-complex is shown to the right. Error bars represent SD; P values were obtained as above. Source data are available for this figure: SourceData F3.

Sro7-NT peptide stimulates exocyst binding to Sec4-GTP but not Snc2. (A) Purified wild-type exocyst binding to immobilized GST-Sec4 beads exchanged with either GTPγS or control GDP in the presence of wild-type Sro7-NT (aa 1–90) peptide or control mutant Sro7-NT-A5,A6. Quantitation of two separate subunits one from each sub-complex is shown to the right. Error bars represent SD. P values were obtained using a two-tailed Student’s t test. *, P <0.05; **, P <0.01; *** P <0.001; ns = no significant difference. (B) Purified exocyst complex binding to immobilized GST-Snc2 or control GST-Snc2E79,E82 beads in the presence of wild-type Sro7-NT (aa 1–90) peptide or control mutant Sro7-NT-A5,A6 peptide. Quantitation of two separate subunits, one from each exocyst sub-complex is shown to the right. Error bars represent SD; P values were obtained as above. Source data are available for this figure: SourceData F3.

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