Y118-Paxillin mutants localize to focal adhesion structures in ZMEL cells in the in vitro cell culture conditions. (A) Western blot revealing relative expression levels of endogenous Paxillin (top panel) and GFP-tagged Paxillin (top panel and middle GFP panel) in ZMEL cells overexpressing mutant and wildtype variants of GFP-tagged Paxillin. Actin is used as a loading control. (B) Representative live images of ZMEL cells expressing GFP-WT/Y118E/Y118F-Paxillin in the in vitro cell culture conditions. Red arrowheads indicate Paxillin-positive focal adhesion structures. Scale bar is 10 µm. Source data are available for this figure: SourceData FS3.