Figure 2. RLC phosphorylation increases... | Rockefeller University Press

RLC phosphorylation increases Ca2+sensitivity of the stress–pCa relationships. (A and B) Ca2+-activated stress–pCa relationships for myocardial strips from WT (A) and cMyBP-C KO (B) mice with AP or MLCK treatment (mean ± SEM). Lines represent three-parameter Hill fits to the average stress–pCa data. (C) Relative RLC phosphorylation quantification by SYPRO Ruby and Pro-Q Diamond. Inset: Representative gel image for AP and MLCK treatment. The number of biological and technical replicates is listed in Table 1. Source data are available for this figure: SourceData F2.

Figure 2.

RLC phosphorylation increases Ca ²⁺ sensitivity of the stress–pCa relationships. (A and B) Ca²⁺-activated stress–pCa relationships for myocardial strips from WT (A) and cMyBP-C KO (B) mice with AP or MLCK treatment (mean ± SEM). Lines represent three-parameter Hill fits to the average stress–pCa data. (C) Relative RLC phosphorylation quantification by SYPRO Ruby and Pro-Q Diamond. Inset: Representative gel image for AP and MLCK treatment. The number of biological and technical replicates is listed in Table 1. Source data are available for this figure: SourceData F2.

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