cMyBP-C loss contributes to interstitial fibrosis. (A and B) Myocardial sections from WT (A) and cMyBP-C KO (B) mice were stained with hematoxylin and eosin to characterize cellular organization and fibrosis. (C) Fibrosis levels were further quantified utilizing a hydroxyproline assay. Averages represent mean ± SEM, with individual data points shown for each heart. Individual data points represent the average of three technical replicates from five biological replicates per genotype.