Laser ablation of an apical bulkhead. Left: Still images from a BC labeled with SiRactin the frame before (–1f), the frame directly after (+1f), and 40 frames after (+40f) ablation of an individual apical bulkhead (see also Video 5). Ablation line is indicated in red, kymograph line is indicated in yellow. Scale bar: 5 µm. Right: Kymograph revealing an immediate local expansion of the BC following ablation of the apical bulkhead and subsequent BC deflation. Red arrowhead indicates moment of laser ablation.