Figure 2.
Apical bulkheads contain a contractile actomyosin cytoskeleton. (a and b) In vitro–differentiated hepatocytes stained for F-actin with phalloidin–AlexaFluor-488 and for non-muscle myosin IIb (a) or phosphomyosin light chain 2 (Ser19; b). Scale bars: 10 µm. (c) 3D reconstruction of the actin cortex (phalloidin, green) and tight junctions (ZO-1, magenta) in a BC segment that was imaged by 3D-STED microscopy (see also Video 1). Scale bar: 2 µm. (d) Single z-slice of a BC segment stained for F-actin with phalloidin–STAR635 and the tight junction marker ZO-1 stained by 3D-STED microscopy. White arrows indicate positions where the subapical actomyosin cortex evaginates into the BC, forming apical bulkhead halves that are sealed by tight junctions. Scale bar: 5 µm.

Apical bulkheads contain a contractile actomyosin cytoskeleton. (a and b) In vitro–differentiated hepatocytes stained for F-actin with phalloidin–AlexaFluor-488 and for non-muscle myosin IIb (a) or phosphomyosin light chain 2 (Ser19; b). Scale bars: 10 µm. (c) 3D reconstruction of the actin cortex (phalloidin, green) and tight junctions (ZO-1, magenta) in a BC segment that was imaged by 3D-STED microscopy (see also Video 1). Scale bar: 2 µm. (d) Single z-slice of a BC segment stained for F-actin with phalloidin–STAR635 and the tight junction marker ZO-1 stained by 3D-STED microscopy. White arrows indicate positions where the subapical actomyosin cortex evaginates into the BC, forming apical bulkhead halves that are sealed by tight junctions. Scale bar: 5 µm.

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