Apical bulkhead halves are connected by adherens junctions and sealed by tight junction loops. (a) Confocal and STED microscopy of a tilted BC between two hepatocytes in vitro stained for F-actin with phalloidin–AlexaFluor-488 (confocal) and the tight junction proteins ZO-1 (STED) and Occludin (STED). Scale bar: 2 μm. (b) STED microscopy of a tilted BC between two hepatocytes in vitro stained for ZO-1 and the adherens junction protein ⍺-catenin. Scale bar: 2 μm.