The average SL value, but not the SL variability, is independent of the optical method used to derive SL striations. Confocal measurements were made either by using SHG microscopy or by fluorescent membrane staining with ANEPPS in n = 26 isolated cardiomyocytes from N = 3 hearts. (A) The median SL value and the following absolute and relative quantitative measures of SL variability were analyzed. (B–E) Inter-quartile range (IQR; B), median absolute deviation (MAD; C), IQR divided by median SL (D), and MAD divided by median SL (E). The data in A–E are shown as mean ± SD. Differences between SHG and ANEPPS were significant at P < 0.0001 (Wilcoxon matched-pairs signed rank test). (F) Averaged distribution plots for individual SL values obtained by SHG or ANEPPS. The peaks of the plots correspond to averaged median SL and the distributions are shown as a percentage of the corresponding median SL.
Figure 2.

The average SL value, but not the SL variability, is independent of the optical method used to derive SL striations. Confocal measurements were made either by using SHG microscopy or by fluorescent membrane staining with ANEPPS in n = 26 isolated cardiomyocytes from N = 3 hearts. (A) The median SL value and the following absolute and relative quantitative measures of SL variability were analyzed. (B–E) Inter-quartile range (IQR; B), median absolute deviation (MAD; C), IQR divided by median SL (D), and MAD divided by median SL (E). The data in A–E are shown as mean ± SD. Differences between SHG and ANEPPS were significant at P < 0.0001 (Wilcoxon matched-pairs signed rank test). (F) Averaged distribution plots for individual SL values obtained by SHG or ANEPPS. The peaks of the plots correspond to averaged median SL and the distributions are shown as a percentage of the corresponding median SL.

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