The comparison of ILEE vs MGT over stability and capability to distinguish biological differences among real biological samples. (a) The coefficient of variation (CV*) of each index rendered by ILEE and MGT by different operators. The individual CV*s of mock and EV group for each index-method combination are merged. ILEE or ILEE_3d got the lowest CV* (or highest stability) on occupancy, linear density, (fluorescence) CV, Diameter_TDT/SDT, segment density, and local anisotropy. (b) A comparison of the t-test P value of mock versus P. syringae (EV)-inoculated sample rendered by ILEE and MGT by different operators. ILEE or ILEE_3d has the highest -log₁₀ P value (i.e., lowest P value) on occupancy, linear density, skewness, (fluorescence) CV, Diameter_SDT, segment density, and anisotropy. Note that the CV* of computed indices in the figure and the fluorescence CV of the samples, which is a cytoskeletal index, are different concept.