The α-Cat M region supports the mechanosensitive recruitment of Jub and Cno. (A) Schematic illustrating protocol for laser ablation experiment to ectopically change tension. Red, actomyosin; pink, TCJs analyzed; cyan, BCJs analyzed; yellow, cytoskeletal force. (B and C) Stills from movie of α-Cat-RNAi α-CatX embryos analyzed, expressing Jub::GFP (B) or Cno::YFP (C). Green spot marks laser cut cell. Arrowhead highlights a TCJ. Scale bar,10 μm. (D) FI of Jub::GFP in BCJs pulled by cut cell after laser ablation. For α-Cat-RNAi α-CatR, n = 14 junctions measured from 7 embryos; α-Cat-RNAi α-Cat-ΔM, n = 14 junctions measured from 7 embryos. (E) FI of Jub::GFP in TCJs of cut cell following laser ablation. For α-Cat-RNAi α-CatR, n = 14 junctions measured from 7 embryos; α-Cat-RNAi α-Cat-ΔM, n = 12 junctions measured from 6 embryos. (F and J) Change in FI at BCJs for Jub::GFP (F) and Cno::YFP (J) in response to increase in tension. Per junction, maximum peak of FI at 10 min of analysis is plotted as a percent increase from minimum in FI at time point of greatest junction length. (G and K) Change in FI at TCJs for Jub::GFP (G) and Cno::YFP (K) in response to increase in tension. Per junction, maximum peak in FI within 4 min of contraction is plotted as a percent increase from minimum in FI in 1 min following maximum cell expansion. (H) FI of Cno::YFP in BCJs pulled by cut cell after laser ablation. For α-Cat-RNAi α-CatR, n = 31 junctions measured from 7 embryos; α-Cat-RNAi α-Cat-ΔM, n = 22 junctions measured from 6 embryos. (I) FI of Cno::YFP in TCJs of cut cell following laser ablation. For α-Cat-RNAi α-CatR, n = 31 junctions measured from 7 embryos; α-Cat-RNAi α-Cat-ΔM, n = 35 junctions measured from 8 embryos. For D, E, H, and I, FI is normalized to correct for photobleaching, curves are aligned by time point of maximum expansion, and smoothed traces are plotted, representing mean and SEM for all junctions analyzed. Pink arrowhead marks peaks in smoothened mean signal. For F, G, J, and K, P values are given by Mann–Whitney tests; gray line, mean, box, SEM, whiskers, SD.
Figure 8.

The α-Cat M region supports the mechanosensitive recruitment of Jub and Cno. (A) Schematic illustrating protocol for laser ablation experiment to ectopically change tension. Red, actomyosin; pink, TCJs analyzed; cyan, BCJs analyzed; yellow, cytoskeletal force. (B and C) Stills from movie of α-Cat-RNAi α-CatX embryos analyzed, expressing Jub::GFP (B) or Cno::YFP (C). Green spot marks laser cut cell. Arrowhead highlights a TCJ. Scale bar,10 μm. (D) FI of Jub::GFP in BCJs pulled by cut cell after laser ablation. For α-Cat-RNAi α-CatR, n = 14 junctions measured from 7 embryos; α-Cat-RNAi α-Cat-ΔM, n = 14 junctions measured from 7 embryos. (E) FI of Jub::GFP in TCJs of cut cell following laser ablation. For α-Cat-RNAi α-CatR, n = 14 junctions measured from 7 embryos; α-Cat-RNAi α-Cat-ΔM, n = 12 junctions measured from 6 embryos. (F and J) Change in FI at BCJs for Jub::GFP (F) and Cno::YFP (J) in response to increase in tension. Per junction, maximum peak of FI at 10 min of analysis is plotted as a percent increase from minimum in FI at time point of greatest junction length. (G and K) Change in FI at TCJs for Jub::GFP (G) and Cno::YFP (K) in response to increase in tension. Per junction, maximum peak in FI within 4 min of contraction is plotted as a percent increase from minimum in FI in 1 min following maximum cell expansion. (H) FI of Cno::YFP in BCJs pulled by cut cell after laser ablation. For α-Cat-RNAi α-CatR, n = 31 junctions measured from 7 embryos; α-Cat-RNAi α-Cat-ΔM, n = 22 junctions measured from 6 embryos. (I) FI of Cno::YFP in TCJs of cut cell following laser ablation. For α-Cat-RNAi α-CatR, n = 31 junctions measured from 7 embryos; α-Cat-RNAi α-Cat-ΔM, n = 35 junctions measured from 8 embryos. For D, E, H, and I, FI is normalized to correct for photobleaching, curves are aligned by time point of maximum expansion, and smoothed traces are plotted, representing mean and SEM for all junctions analyzed. Pink arrowhead marks peaks in smoothened mean signal. For F, G, J, and K, P values are given by Mann–Whitney tests; gray line, mean, box, SEM, whiskers, SD.

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