Cno recruitment to AJs is largely α-Cat-independent. (A) Examples of gastrulating α-Cat-RNAi α-CatX embryos heat-fixed and immunostained for α-CatR or α-CatR-ΔM1 (HA-tagged) and Cno. α-CatR-ΔM1 rescued embryos show a more irregular distribution than α-CatR. Scale bar, 10 μm. (B) Quantification of the colocalization between Cno and α-Cat construct. Significance calculated by Mann-Whitney two-tailed t test (*** = P < 0.001). Bold line shows the median, dotted lines represent interquartile ranges, and n = a pooled number of junctions analyzed (j) from a number of embryos (e). (C) Live imaged examples of Cno::YFP signal in the amnioserosa of α-Cat-RNAi α-CatX embryos; n = number of embryos observed. Scale bar, 10 μm. (D) Ed-RNAi does not change adhesion phenotypes in α-Cat-RNAi α-CatX embryos. Quantification of intact denticle belts; n = number of embryos analyzed. gfp-RNAi controls same as in Fig. 5 E. (E) Cross-section of the lateral ectoderm of an α-Cat-RNAi α-CatR embryo at stage 8 showing that Cno signal is apical to the HA-tagged α-Cat construct.