Planar polarity analysis of AJ proteins in embryos expressing M region deletion constructs in an α-Cat-RNAi background. (A) Plots of the fold changes of the average FI of edges within 15° bins versus that of the 0–15°bin (representing horizontal edges, 0° = anterior–posterior axis). Each dot represents the ratio derived from one embryo. Transparent pink and cyan shapes reflect control and experimental means, respectively, drawn to the height of each bar for ease of comparison. Where overlapping, gray is seen where experimental mean is reduced compared to control pink is visible, and conversely where increased cyan is visible. Significance calculated by ordinary two-way ANOVA with uncorrected Fisher’s LSD for conditions within the same row. (B) Graphs show the mean junctional fluorescent signal per embryo of Ecad along edges in the horizontal or vertical bins in α-Cat-RNAi α-CatX embryos. Significance given by ordinary one-way ANOVA with uncorrected Fisher’s LSD. (C) Enrichment of Vinc FI at vertices linked by three, four, or five junctions per α-Cat-RNAi α-CatX embryos. Significance given by ordinary two-way ANOVA with Tukey’s multiple comparison test. For A–C, n = number of embryos analyzed (e), bar height represents mean, error bars show SD, **** = P < 0.0001, *** = P < 0.001, ** = P < 0.01, * = P < 0.05.